Hormone induced spawning (HCG), and embryonic and larval development of the leopard grouper, Mycteroperca rosacea (Streets, 1877)
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Abstract
Induced spawning of two wild females of the leopard grouper, Mycteroperca rosacea, was obtained using Human Chorionic Gonadotropin (HCG) supplied in two dosages: a first injection of 1000 IU/kg and a second of 500 IU/kg, 24 h apart. Of the four treated females, two had a positive spawning response, releasing approximately 40,000 eggs/fish. These were fertilized with mature male sperm. The fertilization rate obtained for each female was 10% and 0%. Complete embryonic development, and the morphology of eggs and larvae are described until the first feeding. Eggs were spherical, buoyant and transparent, with a diameter of 872.8 ±15.4 ∞m (ranging from 850.4 to 912.5 ∞m) and an oil droplet diameter of 177.3 ± 7.0 ∞m.
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