Production of metabolites from Scenedesmus sp. and a microalgal consortium cultured in unconventional media

Main Article Content

Kevin A González-Falfán
Claudia Guerrero-Barajas
https://orcid.org/0000-0002-4466-2263
Jesús A Badillo-Corona
https://orcid.org/0000-0002-5409-5995
Luis C Fernández-Linares
https://orcid.org/0000-0001-6623-0317

Abstract

Growth comparisons were made between a microalgal consortium and Scenedesmus sp. cultivated in treated wastewater (TWw) enriched with 1 mL·L–1 Bayfolan Forte fertilizer (BM), TWw enriched with (NH4)2HPO4 (PAM), TWw enriched with NH4HCO3 (BCAM), tap water with piggery wastewater (PEM), tap water with piggery wastewater digestate (PDM), and raw wastewater (Ww). Nitrogen (N) content in the media, except for TWw, was adjusted to 80 mg·L–1 N (NH4+-N and NO3-N). Unconventional low-cost media with lower nutrient contents (BM and TWw) showed adequate productions of biomass and lipids. PEM was the most advantageous medium, showing the highest biomass productivity with the consortium (191.25 ± 6.25 g·L–1·d–1) and a lipid productivity of 36.75 ± 9.90 mg·L–1·d–1. The fatty acid profile was composed mainly of C16 and C18. PAM, PEM, and PDM showed a higher proportion of saturated fatty acids (60%–69%), whereas the composition of unsaturated fatty acids was in the range of 31% to 38%. In PEM and PDM the removal of NH4+ was 100%; however, there were NH4+ losses (as NH3) due to volatilization (46%). Unconventional media, especially Ww, are an option for growing microalgae.

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González-Falfán, K. A., Guerrero-Barajas, C., Badillo-Corona, J. A., & Fernández-Linares, L. C. (2021). Production of metabolites from Scenedesmus sp. and a microalgal consortium cultured in unconventional media. Ciencias Marinas, 47(2), 89–103. https://doi.org/10.7773/cm.v47i2.3138
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References

Acién FG, Fernández JM, Magán JJ, Molina E. 2012. Production cost of a real microalgae production plant and strategies to reduce it. Biotechnol Adv. 30(6):1344–1353.

https://doi.org/10.1016/j.biotechadv.2012.02.005

Anand G, Pandey JK, Rana S. 2018. Nanotechnology for Energy and Water: Proceedings of the International Conference NEW-2017; 2017; Dehradun (India). Cham (Switzerland): Springer International Publishing AG.

[APHA] American Public Health Association. 1998. Standard Methods for Examination of Water and Wastewater. Washington, DC: APHA. p. 5–16.

[APHA] American Public Health Association. 2005. Standard Methods for the Examination of Water and Wastewater. Washington, DC: APHA.

Boelee NC, Temmink H, Janssen M, Buisman CJN, Wijffels RH. 2011. Nitrogen and phosphorus removal from municipal wastewater effluent using microalgal biofilms. Water Res. 45(18):5925–5933.

https://doi.org/10.1016/j.watres.2011.08.044

Chen M, Tang H, Ma H, Holland TC, Ng KYS, Salley SO. 2011. Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresour Technol. 102(2):16 49 –1655.

https://doi.org/10.1016/j.biortech.2010.09.062

Chisti Y. 2013. Constraints to commercialization of algal fuels. J Biotechnol. 167(3):201–214.

https://doi.org/10.1016/j.jbiotec.2013.07.020

Dickinson KE, Whitney CG, McGinn PJ. 2013. Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD. Algal Res. 2(2):127–134.

https://doi.org/10.1016/j.algal.2013.01.009

DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem. 28(3):350–356.

https://doi.org/10.1021/ac60111a017

El Shimi HI, Moustafa SS. 2017. Biodiesel production from microalgae grown on domestic wastewater: Feasibility and egyptian case study. 82(3):4238–4244.

https://doi.org/10.1016/j.rser.2017.05.073

Eroglu E, Smith SM, Raston CL. 2015. Appication of various immobilization techniques for algal bioprocesses. In: Moheimani NR, McHenry MP, de Boer K, Bahri PA (eds.), Biofuel and Biorefinery Technologies 2— Biomass and Biofuels from Microalgae. Switzerland: Springer International Publishing. p. 19–44.

Gómez C, Escudero R, Morales MM, Figueroa FL, Fernández-Sevilla JM, Acién FG. 2013. Use of secondary-treated wastewater for the production of Muriellopsis sp. Appl Microbiol Biotechnol. 97(5):2239–2249.

https://doi.org/10.1007/s00253-012-4634 -7

Gonçalves AL, Pires JCM, Simões M. 2017. A review on the use of microalgal consortia for wastewater treatment. Algal Res. 24(B):403–415.

https://doi.org/10.1016/j.algal.2016.11.008

Hernández-García A, Velásquez-Orta SB, Novelo E, Yáñez-Noguez I, Monje-Ramírez I, Orta-Ledesma MT. 2019. Wastewater-leachate treatment by microalgae: Biomass, carbohydrate and lipid production. Ecotox Environ Safe. 174:435–444

https://doi.org/10.1016/j.ecoenv.2019.02.052

Hillier J, Hawes C, Squire G, Hilton A, Wale S, Smith P. 2009. The carbon footprints of food crop production. Int J Agr Sustain. 7(2):107–118.

https://doi.org/10.3763/ijas.2009.0419

Ho S-H, Chen Y-D, Qu W-Y, Liu F-Y, Wang Y. 2019. Algal culture and biofuel production using wastewater. In: Pandey A, Chang JD, Chisti Y (eds.), Biofuels from Algae. 2nd ed. Amsterdam (Netherlands): Elsevier B.V. 603 p.

https://doi.org/10.1016/B978-0-444-64192-2.00008-1

Hu B, Zhou W, Min M, Du Z, Chen P, Ma X, Liu Y, Lei H, Shi J, Ruan R. 2013. Development of an effective acidogenically digested swine manure-based algal system for improved wastewater treatment and biofuel and feed production. Appl Ener. 107:255–263.

https://doi.org/10.1016/j.apenergy.2013.02.033

Huy M, Kumar G, Kim H-W, Kim S-H. 2018. Photoautotrophic cultivation of mixed microalgae consortia using various organic waste streams towards remediation and resource recovery. Bioresource Technol. 247:576–581.

https://doi.org/10.1016/j.biortech.2017.09.10

Ji M-K, Abou-Shanab RAI, Hwang J-H, Timmes TC. 2013. Removal of nitrogen and phosphorus from piggery wastewater effluent using the green microalga Scenedesmusobliquus. J Environ Eng. 139(9):1198–1205.

https://doi.org/10.1061/(asce)ee.1943-7870.0000726

Jia H, Yuan Q. 2016. Removal of nitrogen from wastewater using microalgae and microalgae–bacteria consortia. Cogent Environmental Science. 2(1):1275089.

https://doi.org/10.1080/23311843.2016.1275089

Keeney DR, Nelson DW. 1982. Nitrogen-Inorganic Forms. In: Page AL (ed.), Methods of Soil Analysis, Agronomy Monograph 9, Part 2: Chemical and Microbiological Properties. 2nd ed. Madison (WI): American Society of Agronomy. 643–698.

Lam MK, Yusoff MI, Uemura Y, Lim JW, Khoo CG, Lee KT, Ong HC. 2017. Cultivation of Chlorella vulgaris using nutrients source from domestic wastewater for biodiesel production: Growth condition and kinetic studies. Renew Energ. 103:197–207.

https://doi.org/10.1016/j.renene.2016.11.032

Larsdotter K. 2006. Wastewater treatment with microalgae—A literature review. Vatten. 62:31–38.

Liao Q, Chang J-S, Herrmann C, Xia A. 2018. Bioreactors for microbial biomass and energy conversion. Singapore: Springer Nature.

https://doi.org/10.1007/978-981-10-7677-0

Liu J, Wu Y, Wu C, Muylaert K, Vyverman W, Yu H-Q, Muñoz R, Rittmann B. 2017. Advanced nutrient removal from surface water by a consortium of attached microalgae and bacteria: A review. Bioresource Technol. 241:1127–1137.

https://doi.org/10.1016/j.biortech.2017.06.054

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem. 193(1):265–275.

Lu Y, Xu J. 2015. Phytohormones in microalgae: a new opportunity for microalgal biotechnology? Trends Plant Sci. 20(5):273–282.

https://doi.org/10.1016/j.tplants.2015.01.006

Luo L, He H, Yang C, Wen S, Zeng G, Wu M, Zhou Z, Lou W. 2016. Nutrient removal and lipid production by Coelastrella sp. in anaerobically and aerobically treated swine wastewater. Bioresource Technol. 216:135–141.

https://doi.org/10.1016/j.biortech.2016.05.059

May-Cua ER, Toledano-Thompson T, Alzate-Gaviria LM, Barahona-Perez LF. 2019. A cylindrical-conical photobioreactor and a sludge drying bed as an efficient system for cultivation of the green microalgae Coelastrum sp. and dry biomass recovery. Revista Mexicana de Ingeniería Química. 18(1):1–11.

https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2019v18n1/may

Metz B, Meyer L, Bosch P. 2007. Climate Change 2007: Mitigation of Climate Change. New York (NY): Cambridge University Press. Morales-Amaral MM, Gómez-Serrano C, Acién FG, Fernández-Sevilla JM, Molina-Grima E. 2015. Production of microalgae using centrate from anaerobic digestion as the nutrient source. Algal Res. 9:297–305.

https://doi.org/10.1016/j.algal.2015.03.018

Nam K, Lee H, Hoe S-W, Chang YK, Han JI. 2017. Cultivation of Chlorella vulgaris with swine wastewater and potential for algal biodiesel production. J Appl Phycol. 29(3):1171–1178.

https://doi.org/10.1007/s10811-016-0987-0

Nayak M, Thirunavoukkarasu M, Mohanty RC. 2016. Cultivation of freshwater microalga Scenedesmus sp. using a low-cost inorganic fertilizer for enhanced biomass and lipid yield. J Gen Appl Microbiol. 62(1):7–13.

https://doi.org/10.2323/jgam.62.7

Park J, Jin H-F, Lim B-R, Park K-Y, Lee K. 2010. Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresource Technol. 101(22):8649–8657.

https://doi.org/10.1016/j.biortech.2010.06.142

Park JBK, Craggs RJ, Shilton AN. 2011. Wastewater treatment high rate algal ponds for biofuel production. Bioresource Technol. 102(1):35–42.

https://doi.org/10.1016/j.biortech.2010.06.158

Ramírez-López C, Chairez I, Fernandez-Linares L, 2016. A novel culture medium designed for the simultaneous enhancement of biomass and lipid production by Chlorella vulgaris UTEX 26. Bioresour Technol. 212:207–216.

https://doi.org/10.1016/j.biortech.2016.04.051

Ritchie RJ. 2006. Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth Res. 89(1):27–41.

Rodríguez-Mata AE, Flores-Colunga G, Rangel-Peraza JG, Lizardi-Jiménez MA, Amabilis-Sosa LE. 2019. Estimation of states in photosynthetic systems via chained observers: Design for a tertiary wastewater treatment by using Spirulina maximaon photobioreactor. Revista Mexicana de Ingeniera Quimica. 18(1):273–87.

https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2019v18n1/rodriguez

Sanz-Luque E, Chamizo-Ampudia A, Llamas A, Galvan A, Fernandez E. 2015. Understanding nitrate assimilation and its regulation in microalgae. Front Plant Sci. 6:899.

https://doi.org/10.3389/fpls.2015.00899

Vargas G, Donoso-Bravo A, Vergara C, Ruiz-Filippi G. 2016. Assessment of microalgae and nitrifiers activity in a consortium in a continuous operation and the effect of oxygen depletion. Electron J Biotechn. 23:63–68.

https://doi.org/10.1016/j.ejbt.2016.08.002

Walker HW. 2015. Harmful Algae Blooms in Drinking Water: Removal of Cyanobacterial Cells and Toxins. Boca Raton (FL): CRC Press.Wang M, Yang Y, Chen Z, Chen Y, Wen Y, Chen B. 2016a. Removal of nutrients from undiluted anaerobically treated piggery wastewater by improved microalgae. Bioresource Technol. 222:130–38.

https://doi.org/10.1016/j.biortech.2016.09.128

Wang Y, Ho S-H, Cheng C-L, Guo W-Q, Nagarajan D, Ren N-Q, Lee D-J, Chang J-S. 2016b. Perspectives on the feasibility of using microalgae for industrial wastewater treatment. Bioresource Technol. 222:485–497.

https://doi.org/10.1016/j.biortech.2016.09.106

Wu K-C, Yau Y-H, Ho K-C. 2017. Capability of microalgae for local saline sewage treatment towards biodiesel production. IOP Conf Series. Earth Env Sci. 82:012008.

https://doi.org/10.1088/1755-1315/82/1/012008

Xu J, Zhao Y, Zhao G, Zhang H. 2015. Nutrient removal and biogas upgrading by integrating freshwater algae cultivation with piggery anaerobic digestate liquid treatment. Appl Microbiol Biotechnol. 99(15):6493–6501.

https://doi.org/10.1007/s00253-015-6537-x