Penaeus vannamei challenged with a Vibrio parahaemolyticus AHPND strain shows hepatopancreatic microbiota imbalance

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Lina Angelica Zermeño-Cervantes
Aarón Barraza
Herson Antonio González-Ponce
Sergio Francisco Martinez-Diaz
César Cardona-Félix


White shrimp, Penaeus vannamei, farming represents one of the most important aquaculture activities in the world with a high growth rate. However, intensification processes induce negative side effects on the health of the organism, associated with a dysbiosis phenomenon. Consequently, illnesses, mainly attributable to Vibrio genus bacteria, have been reported in shrimp ponds. Studying the diversity and ecology of the associated bacteria in aquaculture systems is essential to prevent and control diseases. Therefore, the present study analyzes the bacterial load and microbial population variation in P. vannamei hepatopancreases infected with a pathogenic Vibrio parahaemolyticus strain (so-called CVP2) associated with acute hepatopancreatic necrosis disease (AHPND) under controlled conditions. The results showed an important change in the microbial community structure of the P. vannamei hepatopancreas. Furthermore, the presence of the Vibrio genus considerably increased and clearly dominated compared with the control. Dysbiosis of the hepatopancreatic microbiota and constrictions in the hepatopancreatic tubules (characteristic signs of in the early stage of AHPND) could be observed before the visible manifestation of the disease.


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Zermeño-Cervantes, L. A., Barraza, A., González-Ponce, H. A., Martinez-Diaz, S. F., & Cardona-Félix, C. (2023). Penaeus vannamei challenged with a Vibrio parahaemolyticus AHPND strain shows hepatopancreatic microbiota imbalance. Ciencias Marinas, 49.



Ahmed N, Thompson S. 2019. The blue dimensions of aquaculture: A global synthesis. Sci Total Environ. 652:851-861. DOI:

Bonder MJ, Abeln S, Zaura E, Brandt BW. 2012. Comparing clustering and pre-processing in taxonomy analysis. Bioinformatics. 28:2891-2897. DOI:

Cardona E, Gueguen Y, Magré K, Lorgeoux B, Piquemal D, Pierrat F, Noguier F, Saulnier D. 2016. Bacterial community characterization of water and intestine of the shrimp Litopeneaus stylirostris in a biofloc system. BMC Microbiol. 16:157. DOI:

Carrillo-Méndez GJ, Zermeño-Cervantes LA, Venancio-Landeros AA, Díaz-Martinez SF, Cardona-Félix CS. 2019. Natural genetic transformation of Vibrio parahaemolyticus via pVA1 plasmid acquisition as a potential mechanism causing AHPND. Dis Aquat Organ. 137(1):33-40. DOI:

Castañeda LE, Barbosa O. 2017. Metagenomic analysis exploring taxonomic and functional diversity of soil microbial communities in Chilean vineyards and surrounding native forests. PeerJ. 5:e3098. DOI:

Chen W, Zhang CK, Cheng Y, Zhang S, Zhao H. 2013. A comparison of methods for clustering 16S rRNA sequences into OTUs. PLoS ONE. 8:e70837. DOI:

Chen WY, Ng TH, Wu JH, Chen JW, Wang HC. 2017. Microbiome dynamics in a shrimp grow-out pond with possible outbreak of acute hepatopancreatic necrosis disease. Sci Rep. 7:9395. DOI:

Cole JR, Wang Q, Fish JA, Chai B, Mc Garrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR, Tiedje JM. 2014. Ribosomal Database Project: data and tools for high throughput rRNA analysis. Nucleic Acids Res. 42(D1):D633-D642. DOI:

Coleman-Derr D, Desgarennes D, Fonseca-Garcia C, Gross S, Clingenpeel S, Woyke T, North G, Visel A, Partida-Martinez LP, Tringe SG. 2016. Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species. New Phytol. 209:798-811. DOI:

Cornejo-Granados F, Lopez-Zavala AA, Gallardo-Becerra L, Mendoza-Vargas A, Sánchez F, Vichido R, Brieba LG, Viana MT, Sotelo-Mundo RR, Ochoa-Leyva A. 2017. Microbiome of pacific whiteleg shrimp reveals differential bacterial community composition between wild, aquacultured and AHPND/EMS outbreak conditions. Sci Rep. 7:11783. DOI:

Cuéllar-Anjel J. 2014. Métodos para el diagnóstico de enfermedades en camarones Penaeidos. In: Morales V, Cuéllar-Anjel (eds.), Guía técnica - patología e inmunología de camarones Penaeidos. Panamá: OIRSA. 1-52 p.

De Schryver P, Defoirdt T, Sorgeloos P. 2014. Early mortality syndrome outbreaks: a microbial management issue in shrimp farming? PLoS Pathog. 10:e1003919. DOI:

De Souza-Valente C, Wan AHL. 2021. Vibrio and major commercially important vibriosis diseases in decapod crustaceans. J Invertebr Pathol. 181:107527. DOI:

Dong P, Guo H, Wang Y, Wang R, Chen H, Zhao Y, Wang K, Zhang D. 2021. Gastrointestinal microbiota imbalance is triggered by the enrichment of Vibrio in subadult Litopenaeus vannamei with acute hepatopancreatic necrosis disease. Aquaculture. 533:736199. DOI:

Egan S, Gardiner M. 2016. Microbial dysbiosis: rethinking disease in marine ecosystems. Front Microbiol. 7:991. DOI:

Engle CR, McNevin A, Racine P, Boyd CE, Paungkaew D, Viriyatum R, Tinh HQ, Minh HN. 2017. Economics of sustainable intensification of aquaculture: Evidence from shrimp farms in Vietnam and Thailand. J World Aquacult Soc. 48(2):227-239. DOI:

Flegel TW. 2012. Historic emergence, impact and current status of shrimp pathogens in Asia. J Invertebr Pathol. 110(2):166-173. DOI:

Flores-Miranda MC, Luna-González A, Campa-Córdova AI, Fierro-Coronado JA, Partida-Arangure BO, Pintado J, González-Ocampo HA. 2012. Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae). Rev Biol Trop. 60(2):567-576. DOI:

Fu L, Niu B, Zhu Z, Wu S, Li W. 2012. CD-HIT: Accelerated for clustering the next-generation sequencing data. Bioinformatics. 28(23):3150-3152. DOI:

Gao S, Pan L, Huang F, Song M, Tian C, Zhang M. 2019. Metagenomic insights into the structure and function of intestinal microbiota of the farmed Pacific white shrimp (Litopenaeus vannamei). Aquaculture. 499:109-118. DOI:

Gomez-Gil B, Roque A, Rotllant G, Romalde JL, Doce A, Eggermont M, Defoirdt T. 2016. Photobacterium sanguinicancri sp. nov. isolated from marine animals. Anton Van Lee. 109:817-825. DOI:

Gómez-Jiménez S, González-Félix ML, Perez-Velazquez M, Trujillo-Villalba DA, Esquerra-Brauer IR, Barraza-Guardado R. 2005. Effect of dietary protein level on growth, survival and ammonia efflux rate of Litopenaeus vannamei (Boone) raised in a zero water exchange culture system. Aquac Res. 36(9):834-840. DOI:

Han JE, Tang KFJ, Tran LH, Lightner DV. 2015. Photorhabdus insect-related (Pir) toxin-like genes in a plasmid of Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND) of shrimp. Dis Aquat Organ. 113:33-40. DOI:

Hong XP, Xu D, Zhuo Y, Liu HQ, Lu LQ. 2016. Identification and pathogenicity of Vibrio parahaemolyticus isolates and immune responses of Penaeus (Litopenaeus) vannamei (Boone). J Fish Dis. 39(9):1085-1097. DOI:

Jiravanichpaisal P, Miyazaki T, Limsuwan C. 1994. Histopathology, biochemistry, and pathogenicity of Vibrio harveyi infecting black tiger prawn Penaeus monodon. J Aquat Anim Health. 6(1):27-35.<0027:HBAPOV>2.3.CO;2 DOI:<0027:HBAPOV>2.3.CO;2

Johnson CN. 2013. Fitness factors in Vibrios: a mini-review. Microb Ecol. 65(4):826-851. DOI:

Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. 2013. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microb. 79(17):5112-5120. DOI:

Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’hara RB, Simpson GL, Solymos P, Stevens MH, Wagner H. 2014. Vegan: community ecology package. R package version 2.2-0. New Zealand: R project; accessed 2019 March 08. http://CRAN.

Peña-Navarro N, Varela-Mejías A. 2015. Análisis histopatológico en Litopenaeus vannamei infectado con Vibrio parahaemolyticus = Histopathological analysis in Litopenaeus vannamei infected with Vibrio parahaemolyticus. Agron Mesoam. 26:43-53. DOI:

Raja RA, Sridhar R, Balachandran C, Palanisammi A, Ramesh S, Nagarajan K. 2017. Pathogenicity profile of Vibrio parahaemolyticus in farmed pacific white shrimp, Penaeus vannamei. Fish Shellfish Immun. 67:368-381. DOI:

Rognes T, Flouri T, Nichols B, Quince C, Mahé F. 2016. VSEARCH: a versatile open source tool for metagenomics. PeerJ. 4:e2584. DOI:

Rungrassamee W, Klanchui A, Chaiyapechara S, Maibunkaew S, Tangphatsornruang S, Jiravanichpaisal P, Karoonuthaisiri N. 2013. Bacterial population in intestines of the black tiger shrimp (Penaeus monodon) under different growth stages. PLoS ONE. 8:e60802. DOI:

Sirikharin R, Taengchaiyaphum S, Sanguanrut P, Chi TD, Mavichak R, Proespraiwong P, Nuangsaeng B, Thitamadee S, Flegel TW, Sritunyalucksana K. 2015. Characterization and PCR detection of binary, Pir-like toxins from Vibrio parahaemolyticus isolates that cause acute hepatopancreatic necrosis disease (AHPND) in shrimp. PLoS ONE. 10:e0126987. DOI:

Tello M, Valdes N, Vargas R, Rojas J, Parra M, Gajardo G, Gonzalez A. 2019. Application of Metagenomics to Chilean Aquaculture. Metagenomics - Basics, Methods and Applications. United Kingdom: IntechOpen. 162 p. DOI:

Vijay A, Valdes AM. 2022. Role of the gut microbiome in chronic diseases: a narrative review. Eur J Clin Nutr. 76:489-501. DOI:

Wang Q, Garrity GM, Tiedje JM, Cole JR. 2007. Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microb. 73(16):5261-5267. DOI:

Wickham H. 2011. ggplot2: ggplot2. WIREs Comp Stat. 3(2):180-185. DOI:

Zermeño-Cervantes LA, Makarov R, Lomelí-Ortega CO, Martínez-Díaz SF, Cardona-Félix CS. 2018. Recombinant LysVPMS1 as an endolysin with broad lytic activity against Vibrio parahaemolyticus strains associated to acute hepatopancreatic necrosis disease. Aquac Res. 49(4):1723-1726. DOI: