Efecto de un consorcio bacteriano en el crecimiento, morfología, pigmentos y toxicidad del dinoflagelado Gymnodinium catenatum
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Gymnodinium catenatum, un dinoflagelado productor de toxinas paralizantes, es una de las especies de dinoflagelados más estudiadas, sin embargo, poco se conoce sobre sus interacciones con bacterias. En este estudio se determinó el efecto de un consorcio bacteriano en cepas de G. catenatum. Se emplearon 4 cepas de G. catenatum aisladas de Bahía de Santiago, Colima; Lázaro Cárdenas, Michoacán; Bahía Concepción y Bahía de La Paz, Baja California Sur. De la cepa originaria de la costa de Colima se aisló un consorcio bacteriano compuesto de 3 bacterias empleando placas de agar con medio GSe. Se incubaron a una salinidad de 34, a 24 °C y en un ciclo de 12:12 h L:O. Gymnodinum catenatum se cultivó en medio GSe líquido en las mismas condiciones que las bacterias. La interacción entre los microorganismos se evaluó inoculando el consorcio bacteriano en las cepas de G. catenatum. Se determinó la abundancia máxima, las tasas de crecimiento, la longitud de cadenas y el perfil de pigmentos y toxinas paralizantes en G. catenatum en presencia y ausencia del consorcio bacteriano. En presencia de las bacterias no se observó la misma respuesta en el crecimiento de las cepas de G. catenatum, ni en el perfil y contenido de toxinas, pero sí se observó un incremento significativo en el número de células en cadena. También se notaron cambios en la morfología de G. catenatum. Además, en presencia del consorcio bacteriano, en la mayoría de las cepas incrementó el contenido de pigmentos accesorios. Los resultados sugieren que el consorcio bacteriano pudiera ser un agente estresante para G. catenatum proporcionando nuevas perspectivas de interacción entre las bacterias y G. catenatum.
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